FBD-MSP-ZEW for Extraction of Nucleic Acids

Examiner: RDXXX /RDXXX in R&D department; 2016.10.14~10.18; Shanghai Laboratory

1. Examination systems

Clinical specimens: cervical exfoliated cells, a total of four samples

Group B: Dynabeads Myone Magnetic Particles Silane

Group A: centrifuge extraction; Group C: FBD-MSP-ZEW

Methods: the standard protocol for qPCR and nucleic acid extraction

Batches: a total of three batches of FBD-MSP-ZEW tested

2. Results

Part A. extraction efficacy of nucleic acids

                                       Sample 1                                                                                     Sample 2

                                     Sample 3                                                                                          Sample 4

Part B: reproducibility of the sample FBD-MSP-ZEW

                                 Sample 1                                                                                              Sample 2

3. Conclusion

[c1]. FBD-MSP-ZEW had higher efficacy to extract nucleic acids than Dynabeads Myone   Magnetic Particle Silane, both of which were better than the centrifuge extraction.

[c2]. The three batches of FBD-MSP-ZEW gave Ct with CV <5%.

[c3]. FBD-MSP-ZEW has promise for the extraction of nucleic acids in clinical specimens.